Research:
Zinc transport and metabolism, proteomic analysis of biological samples

Teaching:
Micronutrient nutrition

Interests:
Dr. Grider is interested in various aspects of zinc metabolism. Zinc, as an essential trace element, is involved in numerous cellular and biochemical reactions, and has been identified as a structural component or as part of the active site of over 300 different enzymes. Currently, Dr. Grider is focusing on the mechanism(s) involved in the cellular transmembrane transport of this essential trace element using human fibroblast cell lines and a breast epithelial cell line as models. The data accumulated thus far indicates that cell membrane cholesterol is involved in zinc transport, as is anionic exchange, suggesting that noncoated vesicular uptake may be a major mechanism by which zinc is transported into the cell. Dr. Grider is also using two dimensional gel electrophoresis and other proteomic techniques to study proteins which may be involved in zinc transport in a mutant human fibroblast cell line exhibiting abnormal zinc uptake and metabolism. Proteomic techniques in Dr. Grider's lab have also been applied to the study of protein expression in the hippocampus as a function of changes in dietary zinc, as well as to secreted factors from adipose tissue.

Education:

Selected Publications/Presentations:
Mouat, M.F., Greenspan, P., Byerley, L.O., Grider, A. Cholestrol depletion inhibits zinc uptake in MCF-10A cells. Journal of Nutrition (submitted)

Keller, K.A., Grider, A., Coffield, J.A. (2000). Age dependent influence of dietary zinc restriction on short-term memory in male rats. Physiology and Behavior (in press)

Keller, K.A., Chu, Y., Grider, A., Coffield, J.A. (2000). Supplementation with L-histidine during dietary zinc repletion improves short-term memory in zinc-restricted young adult male rats. Journal of Nutrition 130:1633-1640.

Tchoulova, Y., Grider, A., Mouat, M.F., Hausman, G.J. (2000). Priming with magnesium deficient media inhibits preadipocyte differentation via potential upregulation of tumor necrosis factor alpha. Biological Trace Element Research 73:101-111.

Muga, S.J., Grider, A. (1999). Partial characterization of a human zinc-deficiency syndrome by differential display. Biological Trace Element Research 68:1-12.