Research:
Cellular and tissue zinc metabolism; proteomic analysis of biological samples; nutritional biomarkers; plant polyphenol metabolism

Teaching:
FDNS 4100/6100 Micronutrient Nutrition; FDNS 6400 Advanced Macronutrients

Interests:
Dr. Grider is interested in various aspects of zinc metabolism. Zinc, as an essential trace element, is involved in numerous cellular and biochemical reactions, and has been identified as a structural component or as part of the active site of over 300 different enzymes. The research in my laboratory is focused on identifying proteins that are affected by changes in zinc nutriture, and by treatment with other nutrients such as plant polyphenols. Our studies use proteomic techniques such as two dimensional gel electrophoresis and mass spectrometry to determine which proteins are affected by zinc, plant polyphenols, and other nutrients. The results from our studies indicate that changes in zinc nutriture will alter the expression of proteins involved in a number of different pathways, including inflammatory and antioxidant pathways. This information will be useful in understanding the role of zinc, and other nutrients, in biochemical pathways associated with cellular metabolism. Furthermore, the proteins that are identified in these studies may be candidates for biomarkers of zinc and nutrient status.

Education:

Selected Publications/Presentations:

Refereed Publications

Grider, A, Young, EM. (1996) The acrodermatitis enteropathica mutation transiently affects zinc metabolism in human fibroblasts. Journal of Nutrition 126:219-224.
Grider, A, Vazquez, F. (1996) Nystatin affects zinc uptake in human fibroblasts. Biological Trace Element Research 54:97-105.
Chang, CY, Muga, SJ, Grider, A. (1998) Zinc uptake into fibroblasts in inhibited by probenecid. Biochimica et Biophysica Acta 1368:1-6.
Grider, A, Lin, YF, Muga, SJ. (1998) Differences in the cellular zinc content and 5’nucleotidase activity of normal and acrodermatitis enteropathica (AE) fibroblasts. Biological Trace Element Research 61:1-8.
Grider, A, Mouat, MF. (1998) The acrodermatitis enteropathica mutation affects protein expression in human fibroblasts: analysis by two dimensional gel electrophoresis. Journal of Nutrition 128:1311-1314.
Muga, SJ, Grider, A. (1999) Partial characterization of a human zinc-deficiency syndrome by differential display. Biological Trace Element Research 68:1-12.
Keller, KA, Chu, Y, Grider, A, Coffield, JA. (2000) Supplementation with L-histidine during dietary zinc repletion improves short-term memory in zinc-restricted young adult male rats. Journal of Nutrition 130:1633-1640.
Tchoulova, Y, Grider, A, Mouat, MF, Hausman, GJ. (2000) Priming with magnesium deficient media inhibits preadipocyte differentiation via potential upregulation of tumor necrosis factor alpha. Biological Trace Element Research 73:101-111.
Keller, KA, Grider, A, Coffield, JA. (2001) Age dependent influence of dietary zinc restriction on short-term memory in male rats. Physiology and Behavior 72:339-348.
Mouat, MF, Greenspan, P, Byerley, LO, Grider, A. (2003) Zinc uptake into MCF-10A cells is inhibited by cholesterol depletion. Journal of Nutritional Biochemistry 14:74-80.
Chu, Y, Mouat, MF, Coffield, JA, Orlando, R. Grider, A. (2003) The hippocampal expression of P2X6, a purinergic receptor subunit, is affected by dietary zinc deficiency. Biological Trace Element Research 91:77-87.
Chu, Y, Mouat, MF, Harris, RBS, Coffield, JA, Grider, A. (2003) Water maze performance and changes in serum corticosterone levels in zinc-deprived and pair-fed rats. Physiology and Behavior 78:569-578.
Mouat, M.F., Kolli, K., Orlando, R., Hargrove, J.L., Grider, A. (2005) The effects of quercetin on SW480 colon carcinoma cells: a proteomic study. Nutrition Journal. 4:11-17.
Grider, A., Mouat, M.F., Scrimgeour, A.G. (2007) Consumption of a zinc-depleted diet affects soluble protein expression in rat soleus muscle. Journal of Nutritional Biochemistry 18:753-759.
Grider, A., Mouat, M.F., Mauldin, E.A., Casal, M.L. (2007) Analysis of the liver soluble proteome from bull terriers affected with inherited lethal acrodermatitis. Molecular Genetics and Metabolism 92:249-257.
Neal, A.L., Kabengi, N., Grider, A., Bertsch, P.M. (2011) Differing proteomic responses of the soil bacterium Cupriavidus necator to ZnO nanomaterials and aqueous Zn ions. Nanotoxicology DOI:10.3109/17435390.2011.579633.
Phongpa-ngan, P., Grider, A., Mulligan, J.H., Aggrey, S.E., Wicker. L. (2011) Proteomic analysis and differential expression in protein extracted from chicken with varying growth rate and water holding capacity. Journal of Agricultural and Food Chemistry. DOI: 10.1021/jf202622n
Dempsey, C., McCormick, N.H., Kelleher, S.L., Croxford, T.P., Grider, A., Seo, Y.A. (2011) Marginal maternal zinc deficiency in lactating mice redistributes mammary gland zinc pools, reduces secretory capacity and alters milk composition. Journal of Nutrition (in review).

Book Chapters

Grider, A. (2004) Proteomics: Tools for nutrition research in the postgenomic era. In, Genomics and Proteomics in Nutrition. C.D. Berdanier and N. Moustaid-Moussa, eds. Marcel Dekker, Inc. New York. pp. 375-394.
Grider, A. (2006) Chapter 37 Zinc, copper, and manganese. In, Biochemical and Physiological Aspects of Human Nutrition. 2nd edition. M.H. Stipanuk, ed. W.B. Saunders Co., Philadelphia, PA. pp. 1043-1067.
Grider, A. (2012) Chapter 37 Zinc, copper, and manganese. In, Biochemical and Physiological Aspects of Human Nutrition. 3rd edition. M.H. Stipanuk, M.A. Caudill, eds. W.B. Saunders Co., Philadelphia, PA.